Ver

May 18

When I get a clean NMR

confessionsofabadchemist:

image

(via chimiste)

May 17

[video]

officialcomedy: dallonsmiles: YESSSSSSSSS Nightmare fuel.

officialcomedy:

dallonsmiles:

YESSSSSSSSS

Nightmare fuel.

(Source: pj-eckleburg)

May 16

[video]

May 15

motherjones: Pima County, Arizona, is the only county in the United States that tracks migrant deaths. Here’s every one since 2001.

motherjones:

Pima County, Arizona, is the only county in the United States that tracks migrant deaths. Here’s every one since 2001.

May 13

Triple-Decker Motif for Red-Shifted Fluorescent Protein Mutants Bella L. Grigorenko, Alexander V. Nemukhin, Igor V. Polyakov, and Anna I. Krylov Abstract: Among fluorescent proteins (FPs) used as genetically encoded fluorescent tags, the red-emitting FPs are of particular importance as suitable markers for deep tissue imaging. Using electronic structure calculations, we predict a new structural motif for achieving red-shifted absorption and emission in FPs from the GFP family. By introducing four point mutations, we arrive to the structure with the conventional anionic GFP chromophore sandwiched between two tyrosine residues. Contrary to the existing red FPs in which the red shift is due to extended conjugation of the chromophore, in the triple-decker motif, the chromophore is unmodified and the red shift is due to π-stacking interactions. The absorption/emission energies of the triple-decker FP are 2.25/2.16 eV, respectively, which amounts to shifts of 40 (absorption) and 25 nm (emission) relative to the parent species, the I form of wtGFP. Using a different structural motif based on a smaller chromophore may help to improve optical output of red FPs by reducing losses due to radiationless relaxation and photobleaching. Source: http://pubs.acs.org/doi/abs/10.1021/jz4006288 

Triple-Decker Motif for Red-Shifted Fluorescent Protein Mutants

Bella L. Grigorenko, Alexander V. Nemukhin, Igor V. Polyakov, and Anna I. Krylov

Abstract: Among fluorescent proteins (FPs) used as genetically encoded fluorescent tags, the red-emitting FPs are of particular importance as suitable markers for deep tissue imaging. Using electronic structure calculations, we predict a new structural motif for achieving red-shifted absorption and emission in FPs from the GFP family. By introducing four point mutations, we arrive to the structure with the conventional anionic GFP chromophore sandwiched between two tyrosine residues. Contrary to the existing red FPs in which the red shift is due to extended conjugation of the chromophore, in the triple-decker motif, the chromophore is unmodified and the red shift is due to π-stacking interactions. The absorption/emission energies of the triple-decker FP are 2.25/2.16 eV, respectively, which amounts to shifts of 40 (absorption) and 25 nm (emission) relative to the parent species, the I form of wtGFP. Using a different structural motif based on a smaller chromophore may help to improve optical output of red FPs by reducing losses due to radiationless relaxation and photobleaching.

Source: http://pubs.acs.org/doi/abs/10.1021/jz4006288 

(Source: run-away-introvert, via adventures-in-the-lab)

May 12

[video]

dpaf: via

dpaf:

via

(via pricklylegs)

[video]