Ver

cenwatchglass:

Deciphering the Rules of Protein Folding

Chemical & Engineering News, April 10, 1989 [pdf]

From Georgia Tech. It looks like the ones that we used in Chem 183.

Triple-Decker Motif for Red-Shifted Fluorescent Protein Mutants
Bella L. Grigorenko, Alexander V. Nemukhin, Igor V. Polyakov, and Anna I. Krylov
Abstract: Among fluorescent proteins (FPs) used as genetically encoded fluorescent tags, the red-emitting FPs are of particular importance as suitable markers for deep tissue imaging. Using electronic structure calculations, we predict a new structural motif for achieving red-shifted absorption and emission in FPs from the GFP family. By introducing four point mutations, we arrive to the structure with the conventional anionic GFP chromophore sandwiched between two tyrosine residues. Contrary to the existing red FPs in which the red shift is due to extended conjugation of the chromophore, in the triple-decker motif, the chromophore is unmodified and the red shift is due to π-stacking interactions. The absorption/emission energies of the triple-decker FP are 2.25/2.16 eV, respectively, which amounts to shifts of 40 (absorption) and 25 nm (emission) relative to the parent species, the I form of wtGFP. Using a different structural motif based on a smaller chromophore may help to improve optical output of red FPs by reducing losses due to radiationless relaxation and photobleaching.

Source: http://pubs.acs.org/doi/abs/10.1021/jz4006288 

Triple-Decker Motif for Red-Shifted Fluorescent Protein Mutants

Bella L. Grigorenko, Alexander V. Nemukhin, Igor V. Polyakov, and Anna I. Krylov

AbstractAmong fluorescent proteins (FPs) used as genetically encoded fluorescent tags, the red-emitting FPs are of particular importance as suitable markers for deep tissue imaging. Using electronic structure calculations, we predict a new structural motif for achieving red-shifted absorption and emission in FPs from the GFP family. By introducing four point mutations, we arrive to the structure with the conventional anionic GFP chromophore sandwiched between two tyrosine residues. Contrary to the existing red FPs in which the red shift is due to extended conjugation of the chromophore, in the triple-decker motif, the chromophore is unmodified and the red shift is due to π-stacking interactions. The absorption/emission energies of the triple-decker FP are 2.25/2.16 eV, respectively, which amounts to shifts of 40 (absorption) and 25 nm (emission) relative to the parent species, the I form of wtGFP. Using a different structural motif based on a smaller chromophore may help to improve optical output of red FPs by reducing losses due to radiationless relaxation and photobleaching.

Source: http://pubs.acs.org/doi/abs/10.1021/jz4006288 

freshphotons:

Visualizing Size in Biochemical Systems.
backsideattack:

self-organizing map of aryl-amine chemical space
from An investigation into pharmaceutically relevant mutagenicity data and the influence on Ames predictive potential
backsideattack:

the Lego-ization of polyketide biosynthesis

This makes me want to name-drop Prof. Khosla. Whoops, there it is. 

backsideattack:

the Lego-ization of polyketide biosynthesis

This makes me want to name-drop Prof. Khosla. Whoops, there it is. 

chemistry-problems:

Chemistry Problems: Microsoft Word spell checker.
Yes, alkyne is a word. So is phosphodiesterase. And pKa. And stereochemistry. *sigh*

Not a problem for me anymore thanks to this custom chemistry dictionary from ChemistryBlog.

chemistry-problems:

Chemistry Problems: Microsoft Word spell checker.

Yes, alkyne is a word. So is phosphodiesterase. And pKa. And stereochemistry. *sigh*

Not a problem for me anymore thanks to this custom chemistry dictionary from ChemistryBlog.